WebDec 25, 2013 · INTRODUCTION. Single-stranded (ss) DNA binding (SSB) proteins are ubiquitous and found in bacterial, archaeal and eukaryotic cells, mitochondria, phages and viruses ().SSB proteins bind specifically to ssDNA with high affinity in a sequence-independent manner, protect ssDNA from nucleolytic digestion and prevent intrastrand … WebJun 21, 2006 · To study the biochemical properties of single-stranded DNA-binding (SSB) protein from Deinococcus geothermalis (DgeSSB), we have cloned the ssb gene obtained by PCR and developed an overexpression system. The gene consists of an open reading frame of 900 nucleotides encoding a protein of 300 amino acids with a calculated molecular …
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WebOct 1, 2002 · The expression was induced by 0.5 mM IPTG, but it was accompanied by strong inhibition of culture growth.The best induction of thermostable SSB-like proteins … WebTth SSB and Taq SSB have a much lower content of Gly residues than Eco SSB (Table 1). Most of the Gly residues of the thermostable SSB proteins are homologous to conserved Gly in the Eco SSB ... force browser to use ipv6
Protein Expression and Purification - ScienceDirect
WebThis “step up” feature of the SSB reflects the objective of the Savings Bond programme, which is to facilitate long-term savings and investment. Coupon rates for each issuance of Savings Bonds are determined such that the average annual compounded return over an investor’s investment period (e.g. 5 years) is linked to the yield of an SGS ... WebMay 17, 2006 · We have found that the mutant protein of single-stranded DNA binding protein (SSB) from Thermus thermophilus (Tth) HB8 enhances the efficiency of … WebTtAgo is a prokaryotic argonaute from the Gram-negative, thermophilic bacterium Thermus thermophilus which functions as a DNA-guided endonuclease when provided with a 16–18 nucleotide long 5′-phosphorylated single-stranded DNA oligonucleotide guide. The RNase H-like active site of TtAgo requires divalent Mg 2+ (or Mn 2+) (See product usage note #1) … elizabeth brickfield attorney