Galectin puncta assay
WebGalectin. Structure of human galectin-9 in complex with N -acetyllactosamine dimer, clearly showing the two carbohydrate binding sites. Galectins are a class of proteins that bind … WebDec 29, 2024 · To assess the involvement of LMP in cell death induction kinetics, galectin-3 (Gal3) puncta assay was performed using A549 cells stably expressing Aequorea coerulescens green fluorescence protein ...
Galectin puncta assay
Did you know?
WebMay 1, 2024 · Galectins are carbohydrate-binding proteins that are involved in many physiological functions, such as inflammation, immune responses, cell migration, … WebCurrently, the best method for detecting LMP is the galectin puncta formation assay which can be used for a broad range of sample types, both fixed and live, is easy to …
WebFeb 16, 2024 · Munson et al. develop a high-throughput Galectin-9 reporter based endosomal escape imaging assay to probe correlations between nanoparticle-mediated uptake, endosomal escape frequency, and mRNA ... WebJun 26, 2015 · Galectin puncta assay detects LMP in paraffin-embedded tissue samples. (A) Sections of MCF7 breast cancer xenografts collected from SCID/FOX mice 48 h after p.o. treatment with 100 mg/kg ...
Web开馆时间：周一至周日7:00-22:30 周五 7:00-12:00; 我的图书馆 WebNational Center for Biotechnology Information
WebJun 26, 2015 · Here, we characterize and validate the detection of galectin puncta at leaky lysosomes as a highly sensitive and easily manageable …
WebApr 7, 2024 · The confocal images showed that BRD4 forms phase separated puncta in the nuclei of HUVECs which can be ... For network formation assay, approximately 4 × 10 5 cells/well were seeded in six-well plates with ECM overnight, and then starved using DMEM for 12 h. After starvation, replacing DMEM with ECM including different concentrations of … philadelphia dealershipsWebDec 10, 2024 · Finally, the galectin assay sidesteps issues surrounding the subtle dimming of lysosomal puncta upon LMP, characteristic of the dextran and cathepsin assays, by inverting the strategy to assess increased lysosomal puncta in response to LMP. Galectins are a family of cytosolic and secreted lectins that bind to β-galactoside sugars. philadelphia dealsWebJan 6, 2024 · Galectin-3 recruits ESCRT components to damaged lysosomes to repair them ... Sensitive detection of lysosomal membrane permeabilization by lysosomal galectin puncta assay. Autophagy, 11 (2015), pp. 1408-1424. CrossRef View in Scopus Google Scholar. Baietti et al., 2012. philadelphia death toll 2022WebApr 10, 2024 · In vitro infection assay. ... in PBS containing 3% BSA and 0.01% Triton X100 for 1 h. Next, coverslips were incubated with anti- LAMP1 and anti-Galectin-3 at 4 °C overnight. ... Confocal microscopy showed LC3B puncta (green) formation. LC3B puncta formation was quantified and graphically plotted. c Live cell imaging was performed using … philadelphia defamation attorneysWebAug 20, 2024 · The galectin puncta assay can be adapted for use with high-throughput imaging platforms in conjugation with other cell viability markers. 66. Finally, the ESCRT puncta assay used in a recent … philadelphia deals hotelsWebDec 18, 2024 · The detection of galectin puncta at leaky lysosomes as a highly sensitive and easily manageable assay for LMP opens up new possibilities to study LMP in cell death and its role in other cellular processes such as autophagy, senescence, aging, and inflammation. Expand. 231. PDF. philadelphia deed records onlineWebBiological Activity. Autoquin is a novel autophagy inhibitor (IC50=0.56 uM) by indirect modulation of the activity of the lysosomal enzymes acid sphingomyelinase and acid ceramidase; Autoquin showed a dose‐dependent inhibition of EGFP‐LC3 puncta after 3 hours upon autophagy induction by amino acid starvation in the primary screening assay. philadelphia deferred comp nationwide