WebJun 22, 2024 · To overcome this major limitation, we herein report peptide nucleic acid (PNA)-assisted double-stranded DNA nicking by DNAzymes (PANDA) as the first … WebNov 20, 2015 · This DNAzyme was capable of cleaving an all-DNA substrate with appreciable rate constants (k obs ~1.5 × 10 −3 to 0.02 min −1) over a substantial pH range (from 7.2 to 7.7), albeit at the expense of a lower site …
Engineering a DNA-cleaving DNAzyme and PCR into a simple …
WebApr 11, 2024 · The design principle of the Mg 2+-dependent DNAzyme recycling and TdT-mediated DNA polymerization integrated dual amplification for label-free electrochemical detection of MPO is exhibited in Scheme 1.The sensing strategy is composed of a thiolated-hairpin probe (T-HP), a PT-modified hairpin (HP1), and a substrate hairpin (HP2) for Mg … WebDec 1, 2024 · 1. Introduction. Since the first DNAzyme was isolated in 1994 by in vitro selection strategy [1], many DNA catalysts have been developed [[2], [3], [4]].Among them, DNAzymes with cleavage activity are dominant. The 8–17 and 10–23 DNAzymes are typical RNA-cleaving DNA catalysts, originally identified by Joyce group [5].Since then three … sporcle genshin characters
MicroRNA Imaging Encounters Rolling Circle Amplification
To obtain RNA-cleaving DNAzymes that can cleave between any sequence junctions, we used in vitro selection experiments using the substrate 5′-rCrC-3′ (Fig. 1a), because it is considered difficult to cleave among the rPy–rPy junctions. The DNA library with 16-nt randomized sequence was chemically … See more Next, we examined the enzymatic properties of the two DNAzymes. From the time-course cleavage experiments (Fig. 2a), the observed cleavage rates (kobs) of … See more Focusing on ZincDz2 that cleaves between ribocytidines, we undertook a single point mutation analysis on conserved bases in the catalytic core region. For … See more Next, we sought to determine the substrate sequence specificity of ZincDz2. We generated several substrates with different core RNA sequences and compared … See more Finally, to demonstrate the utility of the DNAzyme, we performed cleavage experiments targeting miRNA50, which are functional RNAs of 21–25 bases in … See more WebMar 28, 2014 · The LOD of the this colorimetric method was much lower than the smart mesoporous SiO 2 nanoparticles based fluorescent sensor (50 μM), Zn 2+ -selective DNA-cleaving DNAzyme with PCR strategy (10 ... WebApart from fluorescent beacons, DNAzyme-based colorimetric sensors have been constructed utilizing DNA-functionalized gold nanoparticles (AuNPs). 19 A typical strategy is illustrated in Figure 1.10B, where DNAzymes were used as linkers to assemble the AuNPs forming blue colored aggregates. 80 Upon cleavage, the AuNPs were disassembled … shells fuel and save